Optimizing restriction site placement for synthetic genomes
نویسندگان
چکیده
منابع مشابه
Optimizing Restriction Site Placement for Synthetic Genomes
Restriction enzymes are the workhorses of molecular biology. We introduce a new problem that arises in the course of our project to design virus variants to serve as potential vaccines: we wish to modify virus-length genomes to introduce large numbers of unique restriction enzyme recognition sites while preserving wild-type function by substitution of synonymous codons. We show that the resulti...
متن کاملSynthetic genomes for synthetic biology.
A genome synthesized entirely from scratch has been used to replace the native genome of a living cell, thus creating a new cell. This achievement marks a new frontier in synthetic biology to design and create genomes for organisms with few genetic tools and for applications in areas of energy, health care and the environment.
متن کاملOptimizing Robot Placement for Visit-Point Tasks
We present a manipulator placement algorithm for minimizing the length of the manipulator motion performing a visit-point task such as spot welding. Given a set of points for the tool of a manipulator to visit, our algorithm finds the shortest robot motion required to visit the points from each possible base configuration. The base configuration resulting in the shortest motion is selected as t...
متن کاملComparing Methods for Sentinel Surveillance Site Placement
INTRODUCTION ILI data is collected via an Influenza Sentinel Provider Surveillance Network at the state level. Because participation is voluntary, locations of the sentinel providers may not reflect optimal geographic placement. This study analyzes two different geographic placement schemes – a maximal coverage model (MCM) and a K-median model, two locationallocation models commonly used in geo...
متن کاملSolitary restriction endonucleases in prokaryotic genomes
Prokaryotic restriction-modification (R-M) systems defend the host cell from the invasion of a foreign DNA. They comprise two enzymatic activities: specific DNA cleavage activity and DNA methylation activity preventing cleavage. Typically, these activities are provided by two separate enzymes: a DNA methyltransferase (MTase) and a restriction endonuclease (RE). In the absence of a corresponding...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Information and Computation
سال: 2012
ISSN: 0890-5401
DOI: 10.1016/j.ic.2012.02.003